connexin-43: a possible mediator of heat stress effects on ram sertoli cells
نویسندگان
چکیده
sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. in this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram sertoli cells under mild and severe heat stresses with real-time quantitative pcr. sertoli cells were isolated from testes of 10 lambs. after culture and 3 passages, they were treated with mild (39 ˚c) and severe (42 ˚c) heat stress for 6 hr. the results showed a significant reduction in the percentage of live cells under severe heat stress compared to the control group (32 ˚c), (p
منابع مشابه
Connexin-43: A possible mediator of heat stress effects on ram Sertoli cells
Sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. In this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram Sertoli cells under mild and severe heat stresses with real-time quantitative PCR. Sertoli c...
متن کاملConnexin-43: A possible mediator of heat stress effects on ram Sertoli cells
Sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. In this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram Sertoli cells under mild and severe heat stresses with real-time quantitative PCR. Sertoli c...
متن کاملConnexin 43 and hearing: possible implications for retrocochlear auditory processing.
OBJECTIVES/HYPOTHESIS To examine the relationship between hearing and connexin 43, a dominant gap junctional protein in the central nervous system. STUDY DESIGN Original research. METHODS Connexin 43 heterozygous mice are used to assess its mutational effect on hearing. Results are compared to controls consisting of connexin 43, wild type and CBA/J mice. Hearing is assessed using auditory b...
متن کاملEnhancement of repopulation haemopoiesis by heterozygous connexin 43 stem cells seeded on wild-type connexin 43 stroma.
The present paper analyses the results of competitive blood-cell repopulation experiments in which Cx43-WT (connexin 43 wild-type) host mice, whose own HSCs (haemopoietic stem cells) were deleted, were grafted with fetal liver cells: 50% Gpi-1a (glucose phosphate isomerase-1a)/Cx43-WT cells competing with 50% Gpi-1b/Cx43-WT, 50% Gpi-1b/Cx43-HZ (heterozygous) or 50% Gpi-1b/Cx43-KO (knock-out) ce...
متن کاملRescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43
Perfluorooctanesulfonate (PFOS) is an environmental toxicant used in developing countries, including China, as a stain repellent for clothing, carpets and draperies, but it has been banned in the U.S. and Canada since the late 2000s. PFOS perturbed the Sertoli cell tight junction (TJ)-permeability barrier, causing disruption of actin microfilaments in cell cytosol, perturbing the localization o...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
veterinary research forumناشر: faculty of veterinary medicine, urmia university
ISSN 2008-8140
دوره 6
شماره 2 2015
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023