connexin-43: a possible mediator of heat stress effects on ram sertoli cells

نویسندگان

hossain hassanpour

ali kadivar

pejman mirshokraei

hassan nazari

azita afzali

چکیده

sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. in this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram sertoli cells under mild and severe heat stresses with real-time quantitative pcr. sertoli cells were isolated from testes of 10 lambs. after culture and 3 passages, they were treated with mild (39 ˚c) and severe (42 ˚c) heat stress for 6 hr. the results showed a significant reduction in the percentage of live cells under severe heat stress compared to the control group (32 ˚c), (p

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Connexin-43: A possible mediator of heat stress effects on ram Sertoli cells

Sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. In this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram Sertoli cells under mild and severe heat stresses with real-time quantitative PCR. Sertoli c...

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Connexin-43: A possible mediator of heat stress effects on ram Sertoli cells

Sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. In this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram Sertoli cells under mild and severe heat stresses with real-time quantitative PCR. Sertoli c...

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Connexin 43 and hearing: possible implications for retrocochlear auditory processing.

OBJECTIVES/HYPOTHESIS To examine the relationship between hearing and connexin 43, a dominant gap junctional protein in the central nervous system. STUDY DESIGN Original research. METHODS Connexin 43 heterozygous mice are used to assess its mutational effect on hearing. Results are compared to controls consisting of connexin 43, wild type and CBA/J mice. Hearing is assessed using auditory b...

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Rescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43

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عنوان ژورنال:
veterinary research forum

ناشر: faculty of veterinary medicine, urmia university

ISSN 2008-8140

دوره 6

شماره 2 2015

کلمات کلیدی
[ ' c o n n e x i n ' , 4 3 , ' h e a t s t r e s s ' , ' r a m ' , ' s e r t o l i c e l l ' ]

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